Biological principles and their physical and chemical aspects are explored at the cellular and molecular level. Major emphasis is placed on cell structure, the processes of energy conversion by plant and animal cells, genetics and evolution, and applications to biotechnology.
The structure and function of the cell and its subcellular organelles is studied. Biological macromolecules, enzymes, biomembranes, biological transport, bioenergetics, DNA replication, protein synthesis and secretion, motility, and cancer are covered. Cell biology experiments and interactive computer simulation exercises are conducted in the laboratory.
Introduction to the study of molecular basis of inheritance. Starts with classical Mendelian genetics and proceeds to the study and function of DNA, gene expression and regulation in prokaryotes and eukaryotes, genome dynamics and the role of genes in development, and cancer. All topics include discussions of current research advances. Accompanied by laboratory section that explores the lecture topics in standard wet laboratory experiments and in computer simulations.
This laboratory course introduces essential techniques in molecular biology and genetic engineering in a project format. The course includes aseptic technique and the handling of microbes; isolation and purification of nucleic acids; construction, selection and analysis of recombinant DNA molecules; restriction mapping; immobilization and hybridization of nucleic acids; and labeling methods of nucleic acid probes.
This course is a modern approach to the study of heredity through molecular biology. Primary emphasis is on nucleic acids, the molecular biology of gene expression, molecular recognition and signal transduction, and bacterial and viral molecular biology. The course will also discuss recombinant DNA technology and its impact on science and medicine.
Laboratory practice in modern biological research will be explored. Techniques involving gene and protein cellular probes, ELISA, mammalian cell culturing, cell cycle determination, differential centrifugation, electron microscopy, and fluorescent cellular markets will be addressed. Laboratory fee $60.
Participation in a small group project, under the guidance of a faculty member, whose prior approval is required. Experimentation, application of chemical knowledge and developmental research leading to the implementation of a working chemical process. Individual or group written report required.
Participation in a small group project, under the guidance of a faculty member, whose prior approval is required. Experimentation, application of chemical knowledge and developmental research leading to the implementation of a working chemical process. Individual or group written report required.
Individual research project under the guidance of a chemistry faculty member, whose prior approval is required. A written report in acceptable journal format and an oral presentation are required at the end of the project.
Individual research project under the guidance of a chemistry faculty member, whose prior approval is required. A written report in acceptable journal format and an oral presentation are required at the end of the project.
Systems biology is a new approach to complex biological problems. It uses a combination of the most modern techniques for comprehensive measurements of cells and molecules, combined with complex computer and mathematical modeling, to build up inclusive depictions of how living systems function. This course is an integrative approach to help comprehend dynamic biological systems. True understanding of systems biology requires a cross-disciplinary approach. Topics will include both a biological and computer science perspective taught by experts in each individual discipline. The course will cover introduction to advance biological subjects in cell biology and genetics followed by introduction to computer science methods including modeling and “bio-machine” features of systems biology. In class, we will also explore critical reading of current research.
Schaefer School of Engineering & Science
Department:
Chemistry, Chemical Biology & Biomedical Engineering
Program:
Chemistry & Chemical Biology
Research & Education
Research
The Nuclear Pore Complex and Mitosis
The long-range goal of the Glavy Lab is to outline the molecular mechanisms of the mitotic breakdown of the nuclear pore complex (NPC) with a main focus on the effects of cell cycle dependent modifications on the regulation of nucleoporins (starting with the Nup107-160 subcomplex) and hence the NPC.
The NPC is the principal passageway for nucleocytoplasmic macromolecular traffic. It is composed of about 30 proteins, termed nucleoporins (Nups) designated by their molecular weights in kilodaltons. These are organized into a pseudo-symmetric structure with a two-fold plane quasi-parallel to the nuclear envelope and an eight-fold axis of symmetry across the nucleo-cytoplasmic axis. Most of the Nups are part of a symmetric core structure and therefore occur in at least 16 copies per NPC (e.g. Nup107, Nup133). Others are present only on either the cytoplasmic or the nucleoplasmic side and are present in eight copies per NPC (e.g. Nup358, Nup214, Nup153 also designated FG Nups because of their regional FG (Phe-Gly) sequence repeats). FG Nups are targets of at least nine chromosomal rearrangements found in leukemia, primarily in acute myelogenous leukemia (AML) and myelodysplastic syndrome (MDS). These chromosomal translocations further effect other related Nups, such as Nup107 and Nup133, demonstrating their interdependence which is critical during cell division.
The NPC is disassembled into subcomplexes of Nups during open mitosis in higher eukaryotic cells. Mitotic disassembly is thought to be triggered by phosphorylation. The best characterized subcomplex is the vertebrate Nup107-160 and the homologous Nup84 subcomplex in Saccharomyces cerevisiae. The Nup107-160 subcomplex has 9 members (Nup160, Nup133, Nup107, Nup96, Nup75, Nup43, Nup37, Seh1 and Sec 13. In the “protocoatomer” hypothesis, this nonameric complex has been proposed to stabilize the sharp bend between the inner and outer nuclear envelope membrane.
Postdoctoral Training under Dr. Günter Blobel, Laboratory of Cell Biology, Rockefeller University, NY, NY.
1999: Ph.D. Department of Molecular Pharmacology, Albert Einstein College of Medicine, Yeshiva University, Bronx, NY.
1993: Master of Science. Roswell Park Cancer Institute, Buffalo, NY.
1989: Bachelor of Science. Major: Biology. State University of New York at Buffalo, NY.
Achievements & Professional Societies
Honors & Awards
Harvey N. Davis, Distinguished Teaching Assistant Professor
Selected Publications
Journals
"Selected Recent Publications.....".
Kaur, S., White, T.E., DiGuilio, A.L., Glavy, J.S.. (Aug 1, 2010). "The Discovery of a Werner Helicase Interacting Protein (WHIP) Association with the Nuclear Pore Complex", Cell Cycle, 9 (15), 3106-3111.
Blethrow, J.D., Glavy, J.S. Morgan, D.O., and Shokat K.M.. (2008). "Covalent capture of kinase-specific phosphopeptides reveals Cdk1-cyclin B substrates. ", PNAS, 105 1442-1447.
Glavy, J. S., Krutchinsky, A., Cristea, I.M., Berke, I.C., Boehmer, T., Blobel, G. and Chait, B.T. (2007). "Cell-Cycle Dependent Phosphorylation of the Nuclear Pore Nup107-160 Subcomplex", PNAS, 104 3811-3816.
Book Chapters
Glavy, J.S.. (2009). "Nuclear Pore Complex", The Liver: Biology and Pathobiology. , John Wiley & Sons Press. in press.
Blethrow, J.D. and Glavy, J.S.. (2009). "Purification of Cdk1-Cyclin B Kinase Targeted Phosphopeptides from the Nuclear Envelope", Methods in Molecular Biology: Nuclear Envelope: Methods and Protocols, The Humana Press Inc.. in press.
Presentation/Meeting
Glavy, J. S.. (Jun 15, 2010). "The Discovery of a Werner Helicase Interacting Protein (WHIP) Association with the Nuclear Pore Complex", EMBL Heidelberg, Germany. EMBL Seminar .
The mission of the Department of Chemistry, Chemical Biology, and Biomedical Engineering is to exploit the natural interdependence of science and engineering...read more
