This course extends concepts present in tissue engineering, biotransport and biomaterial to develop design principles for generating tissue and organs in-vitro.  The processes which cells integrate proteins and extracellular matrix to form functioning organ systems are developed.  The principles of bioreactor design are sued to analyze and design in-vitro systems for growing functioning tissue and organs for use as prostheses. Principles of Scale-up to organs of different size are discussed.  Design issues and limitations for extension of these principles to multi-organ systems are illustrated.